dc.contributor.author | Colas, P | fr_FR |
dc.date.accessioned | 2012-08-30T12:31:18Z | |
dc.date.available | 2012-08-30T12:31:18Z | |
dc.date.issued | 2000 | fr_FR |
dc.identifier.citation | Colas, P, Exploration des interactions protéiques à l'échelle génomique., Med Sci (Paris), 2000, Vol. 16, N° 1; p.50-6 | fr_FR |
dc.identifier.issn | 1958-5381 | fr_FR |
dc.identifier.uri | http://hdl.handle.net/10608/1501 | |
dc.description.abstract | L’étude fonctionnelle des
milliers de nouvelles protéines
découvertes grâce aux projets de
séquençage de génomes constitue
un défi scientifique et
technologique majeur. À plus
d’un titre, l’exploration exhaustive
des interactions protéiques
apparaît comme l’une des
approches les plus puissantes de
la génomique fonctionnelle. Cette
revue décrit les différentes
stratégies expérimentales
envisageables reposant sur la
technique du double-hybride. Elle
aborde les problèmes et les
limitations inhérents à cette
technique ainsi que les résultats
attendus et la difficulté de leur
exploitation. Enfin, des méthodes
d’exploration alternatives sont
présentées. | fr |
dc.description.abstract | Genome-scale exploration of protein interactions is one of the most potent approaches of functional genomics. Pioneering efforts have demonstrated that the two-hybrid technique could support such an endeavor. However, the problem of false positive signals remains and needs to be carefully tackled by designing a heuristic to attribute a confidence level for each interaction detected. The expected benefits of building huge databases of protein interactions include insights into the function of many novel proteins, discovery of regulatory pathways or cross-talks between known pathways, structural insights into proteins interactions and possibly analysis of protein interaction patterns. Nevertheless, this approach presents several caveats. Regular two-hybrid assays do not allow the detection of interactions depending on post-translational modifications. Furthermore, information on multi-order protein complexes cannot be accessed easily. In the near future, some of these limitations should be alleviated by a broader use of other methodologies such as mass spectrometry and micro-arrays that may be applied to genome-scale analysis. [References: 26] | en |
dc.language.iso | fr | fr_FR |
dc.publisher | Masson, Paris | fr_FR |
dc.rights | Article en libre accès | fr |
dc.rights | Médecine/Sciences - Inserm - SRMS | fr |
dc.source | M/S. Médecine sciences [revue papier, ISSN : 0767-0974], 2000, Vol. 16, N° 1; p.50-6 | fr_FR |
dc.title | Exploration des interactions protéiques à l'échelle génomique. | fr |
dc.title.alternative | Genome-scale exploration of protein interaction. | fr_FR |
dc.type | Article | fr_FR |
dc.contributor.affiliation | Equipe de Brian B. Rudkin, Laboratoire de biologie moleculaire et cellulaire, UMR 5665, Ecole normale superieure de Lyon, 46, allee d'Italie, 69364 Lyon, France | - |
dc.identifier.doi | 10.4267/10608/1501 | |